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One Step RT-qPCR

NuHi eRT One Step RT-qPCR Probe Kit         catalog#:NH9234   size:20 reaction
NuHi eRT One Step U+ RT-qPCR Probe Kit （anti-contamination）catalog#:NH9248  size:20 reaction

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Product Description

NuHi’s probe-based one step RT-qPCR kit is designed for the detection and quantification of RNA gene expression. This system combines Reverse Transcriptase (RT) and hot-start TaqDNA polymerase in a single enzyme mix. Both cDNA synthesis and PCR are performed in a single tube using gene-specific primers and RNA templates. 

 

Key Features

●  Superior sensitivity——Down to 5 copies/ ul.

    · The high sensitivity enables the detection of low RNA input. 

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※Reference Material Producer：Shanghai Institute of Metrology and Testing Technology


●  Faster & Higher cDNA synthesis temperature

    · The optimal temperature for reverse transcriptase is 55℃ in this system. Higher temperature facilitates reverse         transcription and amplification of templates with secondary structure and high GC contents.

    · The RT reaction can be finished within 10 mins. NuHi’s mix can save 14-34mins per run comparing with other         EUA mix.

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●  High specificity

    · It is common for nonspecific binding to occur when the amplification reaction is set up at room temperature.          Primers can bind to each other, forming primer–dimers. During amplification cycles, primer–dimers can be                extended to produce nonspecific products.

    · However, NuHi’s mix adopted the innovation two phase hot-start mechanism. The RT activity is suppressed           below 37℃ and generates no primer-dimers, which means the specificity can be enhanced greatly.

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※Activity comparison between normal  RT enzyme and hot-start RT enzyme  at 37 ℃


●  Multiplex detection Capable of detecting 4-5 different genes within one assay.

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●  Anti-contamination

    · Adding DUTP and UNG enzyme into system to realize the decontamination at room                           temperature.Compare to the system without UNG, the anti-contamination system shows no loss         in PCR efficiency.

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